KMID : 0608520010070010145
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Korean Journal of Oriental Medicine 2001 Volume.7 No. 1 p.145 ~ p.152
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Discrimination of Aralia continentalis Root by the Random Amplified Polymorphic DNA Analysis and Morphological Characteristics
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À̹̿µ
ÁÖ¿µ½Â/±èÈ«ÁØ/°íº´¼·
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Abstract
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Dried parts of herb medicines are difficult to distinguish morphologically. Heracleum moellendorffii cordata has often been sold instead of Aralia cordata in herbal medicine markets. Therefore, this was conducted to develop the key for discrimination between them using the RAPD analysis and morphological characteristics. Thirty decarmer olihonucleotide primers were screened for the RAPD analysis, and four primers generated distinct RAPD markers specific to Aralia cordata, Angelica prbescens maxim f. biserrata, and Heracleum moellendorffii. The specific RAPD patterns generated by the selected primers were reproducible from dired materials. In comparison of morphological characteristics, Aralia cordata seems to be entirely developed in xylem fiber, but not developed in pith.
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KEYWORD
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Heracleum moellendorffii cordata , Aralia cordata , Angelica bubescens maxim f. biserrata , morphological characteristics , RAPD markers
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